Description
Benzylaminopurine (6-BAP) Powder
Benzylaminopurine (BAP) Powder is a plant growth regulator frequently used in plant tissue culture to encourage cell division and shoot proliferation.
Advantages of Benzylaminopurine (BAP) Powder
- Simple and easy to use.
- Enhances plant growth and development, promotes blossom formation, and boosts fruit production by stimulating cell division.
- Commonly added to tissue culture media, such as MS media.
- Encourages seed germination.
- Supports shoot development in vitro.
- Can also be used to supplement Gamborg’s and Chu’s N6 media.
Steps for Using Benzylaminopurine (BAP) Powder
To prepare tissue culture media with PCT’s BAP powder, follow these steps:
- Prepare liquid tissue culture medium using PCT’s MS, DKW, or another suitable media, depending on the plant’s needs.
- Add sugar according to the volume of media being prepared (typically, 30 grams per liter), along with 1 ml/L PPM™ and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar.
- Mix in the required amount of BAP from the pre-prepared BAP stock solution.
- Sterilize the medium by autoclaving for 20 minutes at 121℃ and 15 psi.
Your tissue culture medium is now ready!
