Description
Malt Extract Yeast Agar Peptone (MYPA)
What is MYPA?
MYPA Mix is an exceptionally nutritious gelatinous medium used for cultivating, isolating, and managing fungi. It features peptone from soybeans, which supports robust mycelium growth and is effective for long-term mushroom culture maintenance (over a year). Applications of MYPA include:
- Expanding mushroom mycelium into grain spawn
- Long-term storage
Why Choose Malt Extract Agar Yeast for Mushroom Cultivation?
Malt Extract
Malt extract, rich in maltose, offers an optimal environment for the growth of yeast and mold due to its ample supply of carbon, protein, and nitrogenous compounds, which serve as energy sources for fungal development.
Agar-Agar
Derived from various seaweeds such as gracilaria and gelidium, Agar-Agar contains no animal ingredients and is prized for its unique gelling properties and stability, making it an essential agent for Petri dish experimentation.
Yeast (S. Cerevisiae)
Cultivated on sugar beet molasses, S. Cerevisiae is a rich source of readily assimilated amino acids and proteins, supplementing mushrooms with dietary fiber, Vitamin D, and B vitamins including Thiamine, Riboflavin, Niacin, Pantothenic Acid, Folic Acid, Biotin, Vitamin B6, and Vitamin B12.
Peptone
Mycological Peptone, derived from Soybean plants, is widely used in liquid culture and agar recipes, as well as added to substrates as a supplement. Peptone supplementation boosts mycelium growth and significantly enhances the quality of the resulting Mycelium Mat.
Not intended for human consumption.
Quantity Specifications:
Measurements for 60*15mm size Petri dishes:
- 21 gm prepares 35 Agar Plates (Use 500ml of hot water)
- 42 gm prepares 70 Agar Plates (Use 1000ml of hot water)
- 63 gm prepares 105 Agar Plates (Use 1500ml of hot water)
- 84 gm prepares 140 Agar Plates (Use 2000ml of hot water)
- 168 gm prepares 280 Agar Plates (Use 4000ml of hot water)
Petri dish not included.
Instructions
Dissolve your MYPA packet in the specified amount of hot water and stir well. For optimal mixing, use a magnetic stirrer. Pour the mixture gradually to prevent air bubbles. Autoclave or pressure cook the mixture for 20 minutes at 15 psi. Pour into Petri dishes at about 50 degrees Celsius and allow to set for 10 minutes without stirring post-autoclave to avoid air bubbles. Once the Petri dishes are set, inoculate them and seal with parafilm. Store the plates aside for a week to monitor for contamination. Store Petri dishes upside down to minimize condensation.
Lacking a pressure cooker? Contact us, and we’ll provide detailed microwave sterilization instructions with your purchase. Please see our listing Make Agar Plates,to find out what you need. Initially, you will need a Reagent Bottle : a 500ml bottle is suitable for microwave use, whereas a 1000ml bottle is needed for use with a sterilizer.
We have extensively used this mix for various experiments in our lab.
Explore our other listings for Pre Mixes. We also offer additional Mushroom-Growing Equipment.
