Description
Create Your Own MYAG Agar Petri Dish Contamination-Free
Elevate your agar plates with Agar Mix MYAG. Antibacterial malt extract yeast agar for precision and purity in your laboratory experiments.
General MYAG with Antibacterial Properties
This is a superior product that is transforming the field of mushroom cultivation. MYAG is a gelatinous medium utilized for purifying, germinating, isolating, and manipulating mushroom mycelium, significantly reducing contamination risks thanks to its antibacterial compound.
Why Use MYAG Instead of Just MYA?
Spores collected from wild, homegrown, or reputable spore sellers often contain bacterial contaminants, a result of spore collection, preservation, and transport methods, such as spore syringes and spore prints. These contaminants can cause rapid bacterial spore growth, which competes with and hampers mushroom spore germination due to their slower growth rate.
However, when spores are introduced to MYAG (with antibacterial properties), the mushroom spores can successfully germinate and grow, effectively leaving bacterial contamination behind. This is because the hyphae—mycelium cells—have long, sharp filaments that help reduce bacterial populations when passing through the antibacterial medium. This antibacterial powder acts like an antibiotic, proven to suppress bacterial levels to manageable extents within agar cultures.
Applications of MYAG with Antibacterial Properties
- Germinating spores from sources like spore syringes or spore prints that may be contaminated.
- Purifying mycelium from other contaminated agar plates.
- Cloning wild mushroom tissue that is likely contaminated.
Quantity specifications:
These quantities are measured based on 60*15mm size Petri dishes:
The number of plates made will vary according to pouring techniques; we recommend using just enough to cover the surface of the petri dish.
- 21 gm makes 35 Agar Plates (Use 500ml of hot water)
- 42 gm makes 70 Agar Plates (Use 1000ml of hot water)
- 63 gm makes 105 Agar Plates (Use 1500ml of hot water)
- 84 gm makes 140 Agar Plates (Use 2000ml of hot water)
- 168gm makes 280 Agar Plates (Use 4000ml of hot water)
Instructions
- Dissolve your MYAG packet in the specified ratio of hot water (i.e.1000, ml water for 42 gm of MYAG) and stir thoroughly with a spoon. If you have a magnetic stirrer, this will mix the solution properly. The content needs to be poured slowly and not all at once.
- Autoclave or pressure cook for 15 minutes at 15 psi.
- Pour into Petri dishes when it reaches 50 degrees Celsius (Approximately hot enough to hold the flask/ jar with one hand), and let it set for 10 minutes. Do not stir the solution after taking it out of Autoclave, as this would trap air bubbles in this solution.
- Once the Petri dishes are set, inoculate them and wrap them with parafilm. Keeping the plates aside for a week is recommended to check for contamination.
- Petri dishes are best to be stored upside down to avoid condensation.
No Pressure Cooker? No Problem!
Message us and we’ll provide detailed microwave sterilization instructions with your purchase. Please see our listing Make Agar Plates,to find out what you need. Initially, you will need a Reagent Bottle : a 500ml bottle is suitable for microwave use, whereas a 1000ml bottle is needed for use with a sterilizer.
We have extensively used this mix to conduct various experiments in our lab.
Explore our other listings for Pre Mixes. We also offer additional Mushroom-Growing Equipment.
You can also purchase antibacterial powder from our store for your specialized needs.
