Description
Malt Extract Yeast Agar (MYA)
What is MYA?
Malt Extract Agar Mix is a popular gelatinous medium employed for cultivating, isolating, and manipulating fungi, yeast, and molds. It serves as a vital method for the prolonged maintenance of mushroom cultures. Key applications of MYA include:
- Germination of spores
- Isolation of specific mushroom strains
- Cultivation of mushroom mycelium for transfer to grain spawn
- Assessment of liquid culture contamination
- Long-term storage of cultures
Why Choose Malt Extract Agar Yeast for Mushroom Cultivation?
Malt Extract
Rich in maltose, malt extract provides an optimal environment conducive to the growth of yeast and mold, thanks to its abundant carbon, protein, and nitrogenous resources that fuel fungal development.
Agar-Agar
Derived from seaweeds such as gracilaria and gelidium, Agar-Agar is free from animal ingredients. Its unique gelling properties and stability make it an ideal firming and thickening agent in Petri dish experimentation.
Yeast (S. Cerevisiae)
Cultivated on sugar beet molasses, the S. Cerevisiae strain is a potent source of easily absorbed amino acids and proteins, enriching mushrooms with dietary fiber, Vitamin D, and essential B Complex vitamins.
Note: MYA differs from MEA, as it includes Yeast while MEA comprises only Malt Extract and Agar.
For more information, visit our section on Pre-Poured Agar Plates and become an expert.
Quantity Specifications:
Based on 60*15mm Petri dishes, the output varies with pouring techniques; ideally, enough to just cover each dish surface.
- 21 gm produces 35 Agar Plates (Use 500ml of hot water)
- 42 gm produces 70 Agar Plates (Use 1000ml of hot water)
- 63 gm produces 105 Agar Plates (Use 1500ml of hot water)
- 84 gm produces 140 Agar Plates (Use 2000ml of hot water)
- 168 gm produces 280 Agar Plates (Use 4000ml of hot water)
Petri dishes not included.
Instructions
Dissolve the MYA mix in the indicated amount of hot water and mix thoroughly. Use a magnetic stirrer for optimal results. Pour the mixture gradually to avoid air bubble formation. Autoclave or pressure cook the mixture at 15 psi for 20 minutes. Pour into Petri dishes at about 50 degrees Celsius and allow to set for 10 minutes without stirring post-autoclave to prevent air bubbles. After setting, inoculate the dishes and seal with parafilm. Set aside for a week to monitor for contamination. Store the Petri dishes upside down to minimize condensation.
Don’t have a pressure cooker? No worries; message us and we’ll provide detailed microwave sterilization instructions with your purchase. Please see our listing Make Agar Plates,to find out what you need. Initially, you will need a Reagent Bottle : a 500ml bottle is suitable for microwave use, whereas a 1000ml bottle is needed for use with a sterilizer.
Rely on this mix for diverse experiments in our lab.
Explore our other listings for Pre Mixes. We also offer additional Mushroom-Growing Equipment.
